Step 1

Please Select 3. Taxonomic Analysis on the top bar.

Step 2

This process results in a prediction of the possible taxa based on the database (Silva or Greengene).
After you download the database to the specified directory, please select the database type you want.

Step 3

Please input the primer sequences to extract the target region from database sequences.

If your primers cannot not be found in the selections, you can manually input your primer sequences by choosing “Other”.

If you click on the button “Show the primer table”, the primer table will be displayed.

Step 4

In this step, the sequences will be filter based on the length.

Sequences shorter than the minimun length will be discarded, and the sequences longer than the maximun length will be discarded.

Step 5

Determine how many the threads you want to use for the process

Step 6

Click on the button Start to analyze to start the process.

Step 7

Click on the button “taxonomy result” to inspect the predicted taxonomy.

If you would like to know what the result look likes, please click on the button “How does the taxonomy look?” in the example block.